The use of environmental DNA (eDNA) is a relatively new indirect method of species detection. It offers several advantages over traditional detection techniques such as its sensitivity and that it does not require capture, handling or direct observation of the species of interest. Despite the rising interest on utilising eDNA and its potential application to many areas of wildlife research, there are a number of factors that can impact upon the limits of eDNA detection and these are still relatively unknown for most species. These factors include the persistence of eDNA in water, the impacts of environmental conditions (e.g. temperature, UV light, PH, flow rates), and where and when to sample. The practical applicability and adoption of eDNA as a standard tool in monitoring requires a quantitative framework that provides a probability of detection, and must also be robust and cost-effective in comparison with traditional wildlife detection methods. In this poster presentation, I will present our preliminary findings comparing in-situ eDNA detection of the Oriental weatherloach (Misgurnus anguillicaudatus) with standard fish sampling methods.  I will also outline the various approaches we will take to improve detection probabilities for rare species using eDNA.